Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line

Proc Natl Acad Sci U S A. 1986 Jul;83(14):5010-4. doi: 10.1073/pnas.83.14.5010.


Among a series of myeloid leukemia cell lines, one (NFS-60) was found to have a rearrangement of the c-myb locus. The rearrangement involved the integration of a retrovirus into the region of the gene corresponding to the sixth exon of the avian c-myb locus. The insertion is associated with the production of a truncated RNA and the introduction of a terminator codon at the juncture of the long terminal repeat and the c-myb locus. The properties of the NSF-60 cells were compared with those of other myeloid cell lines, and the known sequence of differentiation induced by interleukin 3. Similar to other myeloid cell lines, the NFS-60 cells do not terminally differentiate in response to interleukin 3, granulocyte/macrophage, or granulocyte colony-stimulating factor suggesting that the cells are transformed with regard to their ability to differentiate. The NFS-60 cells are totally dependent on interleukin 3 for growth and maintenance of viability in vitro but also proliferate in response to granulocyte colony-stimulating factor. The properties of the cells support the concept that the c-myb protooncogene is involved in the control of normal differentiation of hematopoietic cells.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Cell Line
  • DNA, Neoplasm / analysis*
  • DNA, Viral / analysis*
  • Interleukin-3
  • Leukemia Virus, Murine / genetics*
  • Leukemia, Experimental / genetics*
  • Leukemia, Myeloid / genetics*
  • Lymphokines / pharmacology
  • Mice
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-myb
  • RNA, Messenger / analysis
  • RNA, Neoplasm / analysis


  • DNA, Neoplasm
  • DNA, Viral
  • Interleukin-3
  • Lymphokines
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-myb
  • RNA, Messenger
  • RNA, Neoplasm

Associated data

  • GENBANK/K03547
  • GENBANK/M13989
  • GENBANK/M13990