Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs

Proc Natl Acad Sci U S A. 1986 Jul;83(14):5057-61. doi: 10.1073/pnas.83.14.5057.


The Escherichia coli mutS gene product is involved in mismatch correction in this organism. We have purified a biologically active form of the 97,000 Mr protein to near homogeneity from an overproducing strain. Enzymatic and chemical protection ("footprinting") experiments have demonstrated that mutS-encoded protein specifically binds to DNA regions containing a single base-pair mismatch. The protein displayed variable affinity for the limited set of mismatches tested (G-T greater than G-A approximately equal to A-C greater than T-C).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Base Composition
  • DNA Repair*
  • DNA, Bacterial / metabolism*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • Nucleic Acid Conformation
  • Protein Binding
  • Substrate Specificity


  • Bacterial Proteins
  • DNA, Bacterial
  • DNA-Binding Proteins