A parallel demonstration of different resins' antibody aggregate removing capability by a case study

Protein Expr Purif. 2019 Jan:153:59-69. doi: 10.1016/j.pep.2018.08.011. Epub 2018 Aug 23.

Abstract

Aggregation is a major concern for therapeutic monoclonal antibody (mAb), as aggregates reduce drug efficacy and safety. In addition to aggregation control, aggregate removal by downstream processing is crucial. Hydrophobic and mixed-mode resins are widely used for aggregate removal in different cases, but they are seldom compared side by side. In this study, the aggregate removing capability of eight resins belonging to different chromatographic types was demonstrated by a case study. This work, by providing multiple options for aggregate removal, allows more flexibility to be gained in downstream processing.

Keywords: Aggregate removal; Antibody aggregate; Hydrophobic interaction chromatography (HIC); Hydroxyapatite (HA); Mixed-mode resin; Polyethylene glycol (PEG).

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / isolation & purification*
  • CHO Cells
  • Chromatography, Ion Exchange / instrumentation
  • Chromatography, Liquid / instrumentation
  • Cricetulus
  • Durapatite / chemistry*
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Ion Exchange Resins / chemistry*
  • Polyethylene Glycols / chemistry
  • Protein Aggregates*
  • Resins, Synthetic / chemistry*

Substances

  • Antibodies, Monoclonal
  • Ion Exchange Resins
  • Protein Aggregates
  • Resins, Synthetic
  • Polyethylene Glycols
  • Durapatite