Abstract
Mammalian cell lines have been engineered to produce a secreted form of the AIDS retrovirus envelope glycoprotein. The recombinant protein has been isolated from growth-conditioned culture media and used to immunize animals. Antibodies directed against the recombinant molecule were found to react with the envelope glycoprotein produced in virus-infected cells. Furthermore, these antibodies were able to directly inactivate the AIDS retrovirus in a neutralization assay in vitro. The expression system reported here should provide sufficient quantities of the AIDS retrovirus envelope protein for biological and vaccination studies.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Acquired Immunodeficiency Syndrome / immunology*
-
Animals
-
Antibodies, Viral / immunology*
-
Antigens, Viral / biosynthesis
-
Antigens, Viral / immunology*
-
Cricetinae
-
Deltaretrovirus / immunology*
-
Guinea Pigs
-
HIV Antibodies
-
HIV Antigens
-
Humans
-
Immune Sera / immunology
-
Male
-
Neutralization Tests
-
Rabbits
-
Recombinant Proteins / biosynthesis
-
Recombinant Proteins / immunology
-
Viral Envelope Proteins / biosynthesis
-
Viral Envelope Proteins / immunology*
-
Viral Vaccines / immunology
Substances
-
Antibodies, Viral
-
Antigens, Viral
-
HIV Antibodies
-
HIV Antigens
-
Immune Sera
-
Recombinant Proteins
-
Viral Envelope Proteins
-
Viral Vaccines