The ameliorative effect of hemp seed hexane extracts on the Propionibacterium acnes-induced inflammation and lipogenesis in sebocytes

PLoS One. 2018 Aug 27;13(8):e0202933. doi: 10.1371/journal.pone.0202933. eCollection 2018.

Abstract

In this study, we investigated the anti-microbial, anti-inflammatory, and anti-lipogenic effects of hemp (Cannabis sativa L.) seed hexane extracts, focusing on the Propionibacterium acnes-triggered inflammation and lipogenesis. Hemp seed hexane extracts (HSHE) showed anti-microbial activity against P. acnes. The expression of iNOS, COX-2, and the subsequent production of nitric oxide and prostaglandin increased after infection of P. acnes in HaCaT cells, however, upon treating with HSHE, their expressions were reduced. P. acnes-induced expressions of IL-1β and IL-8 were also reduced. HSHE exerted anti-inflammatory effects by regulating NF-κB and MAPKs signaling and blunting the translocation of p-NF-κB to the nucleus in P. acnes-stimulated HaCaT cells. Moreover, P. acnes-induced phosphorylation of ERK and JNK, and their downstream targets c-Fos and c-Jun, was also inhibited by HSHE. In addition, the transactivation of AP-1 induced by P. acnes infection was also downregulated by HSHE. Notably, HSHE regulated inflammation and lipid biosynthesis via regulating AMPK and AKT/FoxO1 signaling in IGF-1-induced inflammation and lipogenesis of sebocytes. In addition, HSHE inhibited 5-lipoxygenase level and P. acnes-induced MMP-9 activity, and promoted collagen biosynthesis in vitro. Thus, HSHE could be utilized to treat acne vulgaris, through its anti-microbial, anti-inflammatory, anti-lipogenic, and collagen-promoting properties.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / isolation & purification
  • Anti-Bacterial Agents / pharmacology*
  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / pharmacology*
  • Arachidonate 5-Lipoxygenase / chemistry
  • Cannabis / chemistry*
  • Cells, Cultured
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Gram-Positive Bacterial Infections / drug therapy
  • Gram-Positive Bacterial Infections / microbiology
  • Hexanes / chemistry
  • Humans
  • Inflammation / drug therapy*
  • Inflammation / etiology
  • Inflammation / pathology
  • Keratinocytes / cytology
  • Keratinocytes / drug effects
  • Keratinocytes / metabolism
  • Lipogenesis / drug effects*
  • Lipoxygenase Inhibitors / isolation & purification
  • Lipoxygenase Inhibitors / pharmacology
  • Plant Extracts / pharmacology*
  • Propionibacterium acnes / drug effects*
  • Propionibacterium acnes / pathogenicity
  • Sebaceous Glands / cytology
  • Sebaceous Glands / drug effects*
  • Sebaceous Glands / metabolism
  • Seeds / chemistry

Substances

  • Anti-Bacterial Agents
  • Anti-Inflammatory Agents
  • Hexanes
  • Lipoxygenase Inhibitors
  • Plant Extracts
  • Arachidonate 5-Lipoxygenase

Grant support

This work was supported by Soonchunhyang University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.