Multi-enzyme complex of white rot fungi in saccharification of lignocellulosic material

Wilton Soares Cardoso; Paula Viana Queiroz; Gabriella Peterlini Tavares; Fernando Almeida Santos; Filippe Elias de Freitas Soares; Maria Catarina Megumi Kasuya; José Humberto de Queiroz

doi:10.1016/j.bjm.2018.05.006

Multi-enzyme complex of white rot fungi in saccharification of lignocellulosic material

Braz J Microbiol. 2018 Oct-Dec;49(4):879-884. doi: 10.1016/j.bjm.2018.05.006. Epub 2018 Aug 14.

Authors

Wilton Soares Cardoso¹, Paula Viana Queiroz², Gabriella Peterlini Tavares³, Fernando Almeida Santos⁴, Filippe Elias de Freitas Soares³, Maria Catarina Megumi Kasuya⁵, José Humberto de Queiroz³

Affiliations

¹ Instituto Federal do Espírito Santo, Venda Nova do Imigrante, ES, Brazil. Electronic address: wilton.cardoso@ifes.edu.br.
² Universidade Federal de Viçosa, Departamento de Química, Viçosa, MG, Brazil.
³ Universidade Federal de Viçosa, Departamento de Bioquímica, Viçosa, MG, Brazil.
⁴ Universidade Estadual do Rio Grande do Sul, Departamento de Bioenergia, Porto Alegre, RS, Brazil.
⁵ Universidade Federal de Viçosa, Departamento de Microbiologia, Viçosa, MG, Brazil.

Abstract

The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commercial enzyme. Most fungi complexes had better hydrolysis rates compared with purified commercial enzyme.

Keywords: Cellulases; Enzymes; Fungus; Hydrolysis.

MeSH terms

Biocatalysis
Cellulases / chemistry*
Cellulases / metabolism
Fungal Proteins / chemistry*
Fungal Proteins / metabolism
Fungi / chemistry
Fungi / enzymology*
Hydrolysis
Lignin / chemistry*
Lignin / metabolism
Plant Stems / chemistry
Plant Stems / microbiology
Sorghum / chemistry*
Sorghum / microbiology

Substances

Fungal Proteins
lignocellulose
Lignin
Cellulases