Fermented Lingonberry Juice Inhibits Oral Tongue Squamous Cell Carcinoma Invasion In Vitro Similarly to Curcumin

In Vivo. Sep-Oct 2018;32(5):1089-1095. doi: 10.21873/invivo.11350.

Abstract

Background: Oral tongue squamous cell carcinoma (OTSCC) cells are highly proliferative and invasive. Lingonberry contains several polyphenolic compounds similar to curcumin. We hypothesize that fermented lingonberry juice (FLJ) has an anti-invasive and anti-proliferative effect on OTSCC cells similarly to curcumin, which is known to be anti-carcinogenic.

Materials and methods: FLJ, curcumin dissolved in ethanol, or curcumin loaded in Candida extracellular vesicles (EVs) were added to more (HSC-3) and less aggressive (SCC-25) OTSCC cells. Cell proliferation was measured with a 5-bromo-2'-deoxyuridine kit and invasion in the three-dimensional Myogel spheroid assay. Statistical analyses were completed with one-way ANOVA and Bonferroni post-hoc testing.

Results: Both FLJ and curcumin significantly reduced the proliferation and invasion of HSC-3 and SCC-25 cells. The effects of curcumin were not improved when cells were treated with curcumin loaded within EVs.

Conclusion: Our results suggest that FLJ, like curcumin, has an anti-carcinogenic effect on aggressive OTSCC cells in vitro.

Keywords: 3D spheroid; Oral cancer cells; anti-invasive; anti-proliferative.

MeSH terms

  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Carcinoma, Squamous Cell
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Curcumin / pharmacology*
  • Dose-Response Relationship, Drug
  • Fruit and Vegetable Juices*
  • Humans
  • Mouth Neoplasms
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Tumor Cells, Cultured
  • Vaccinium vitis-idaea / chemistry*

Substances

  • Antineoplastic Agents, Phytogenic
  • Plant Extracts
  • Curcumin