Identification of novel OXA-134-like β-lactamases in Acinetobacter lwoffii and Acinetobacter schindleri isolated from chicken litter

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019 Jun;163(2):141-146. doi: 10.5507/bp.2018.037. Epub 2018 Jul 18.


Background: Various food-producing animals have been recognized in recent years as a potential reservoir for the spread of antibiotic resistant bacteria that may pose a risk to human health and therefore their dissemination in the food production chain needs to be assessed.

Methods: In this study, 450 boot swabs from chicken farms were analyzed for the presence of antimicrobial resistance with a focus on β-lactams resistance in Acinetobacter species.

Results: Two β-lactamase-encoding genes were first time identified in Acinetobacter lwoffii and Acinetobacter schindleri isolates. The deduced amino acid sequence of OXA-496 shared 93.8% identity with OXA-363. The second OXA-134-like enzyme, OXA-537, had the highest sequence identity (97.8%) with OXA-235 and OXA-237.

Conclusions: The results of this study illustrate the occurrence of new OXA-134-like β-lactamases, called OXA-496 and OXA-537, carrying strains of Acinetobacter lwoffii and Acinetobacter schindleri in chicken farm litter, and highlight the possible role of Acinetobacter as a reservoir of resistance genes.

Keywords: antibiotics; chicken; identification; resistance; β-lactamase.

MeSH terms

  • Acinetobacter / genetics*
  • Animals
  • Chickens
  • Czech Republic
  • Drug Resistance, Bacterial / genetics
  • Farms
  • Microbial Sensitivity Tests
  • beta-Lactam Resistance / genetics*
  • beta-Lactamases / genetics*


  • beta-Lactamases

Supplementary concepts

  • Acinetobacter lwoffii
  • Acinetobacter schindleri