Electroporation allows targeting of genetic materials (e.g., DNA, RNA, antisense morpholinos) to the tissue of interest with high spatial and temporal specificity. Here, we describe a highly efficient and reproducible electroporation strategy for targeting the central nervous system in Xenopus. This versatile approach can be combined with live imaging or other existing experimental procedures to aid the investigation of different research questions.
Keywords: Axon navigation; Cell Imaging; Electroporation; Fluorescence; In vivo imaging; Living imaging; Neural wiring; Neurodevelopment; Optic tract; Xenopus.