Manipulating and Analyzing Cell Type Composition of the Xenopus Mucociliary Epidermis

Methods Mol Biol. 2018;1865:251-263. doi: 10.1007/978-1-4939-8784-9_18.

Abstract

The Xenopus embryonic epidermis serves as a model to investigate the development, cell biology, and regeneration of vertebrate mucociliary epithelia. Its fast development as well as the ease of manipulation and analysis in this system facilitate novel approaches and sophisticated experiments addressing the principle mechanisms of mucociliary signaling, transcriptional regulation, and morphogenesis. This protocol describes how cell type composition can be manipulated and analyzed, and how mucociliary organoids can be generated and used for "omics"-type of experiments.

Keywords: Cilia; Epidermis; Goblet cell; Ionocyte; Mucociliary; Multiciliated cell; Small secretory cell; Xenopus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cilia / metabolism*
  • Embryo, Nonmammalian / metabolism
  • Epidermis / metabolism*
  • Molecular Biology / methods*
  • Mucus / metabolism*
  • Organoids / metabolism
  • Xenopus laevis / embryology
  • Xenopus laevis / metabolism*