Cohabitation of scaffold binding regions with upstream/enhancer elements of three developmentally regulated genes of D. melanogaster

Cell. 1986 Aug 15;46(4):521-30. doi: 10.1016/0092-8674(86)90877-9.


We find DNA fragments attached to the nuclear scaffold (SARs) both 5' and 3' of three Drosophila genes, defining looped domains ranging from 4.5 to 13 kb. For the two-promoter-containing gene Adh (alcohol dehydrogenase), we find two upstream and two downstream SARs. For Sgs-4, the 5' SAR covers 866 bp immediately upstream of the transcript, and in the case of fushi tarazu, the 5' SAR is found on a small fragment 4.8 kb upstream of the start of transcription. These four upstream scaffold-attached fragments comap with enhancer-like regulatory sequences. Sequence analysis of five upstream SARs reveals clusters of sequences closely related to the cleavage consensus of topoisomerase II, several copies of a specific 10 bp A-rich sequence (AATAAATCAAA), and another 10 bp T-rich stretch.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Dehydrogenase
  • Alcohol Oxidoreductases / genetics
  • Animals
  • Binding Sites
  • Cell Nucleus / ultrastructure
  • Chromatin / ultrastructure*
  • DNA Topoisomerases, Type I / metabolism
  • DNA-Binding Proteins / metabolism
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / growth & development
  • Enhancer Elements, Genetic*
  • Genes
  • Genes, Regulator*
  • Larva
  • Morphogenesis
  • Promoter Regions, Genetic
  • Proteins / genetics
  • Salivary Proteins and Peptides / genetics


  • Chromatin
  • DNA-Binding Proteins
  • Proteins
  • Salivary Proteins and Peptides
  • Alcohol Oxidoreductases
  • Alcohol Dehydrogenase
  • DNA Topoisomerases, Type I