Direct regional assignment of the gene for vitamin D binding protein (Gc-globulin) to human chromosome 4q11-q13 and identification of an associated DNA polymorphism

Hum Genet. 1986 Jul;73(3):225-9. doi: 10.1007/BF00401232.

Abstract

Using a characterized human vitamin D binding protein (DBP) cDNA probe and a panel of rodent X human somatic cell hybrids, we established the chromosomal location of the structural gene for DBP on human chromosome 4. In situ hybridization of 3H-labeled DBP cDNA to human metaphase chromosomes confirmed this assignment and allowed regional localization to bands 4q11-4q13. A restriction fragment length polymorphism associated with the DBP gene that should prove useful in future linkage studies was identified with the enzyme BamHI.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Chromosome Banding
  • Chromosome Mapping*
  • Chromosomes, Human, 4-5*
  • Cricetinae
  • DNA / genetics
  • DNA Restriction Enzymes
  • Deoxyribonuclease BamHI
  • Genes*
  • Genetic Linkage
  • Humans
  • Hybrid Cells
  • Karyotyping
  • Polymorphism, Genetic
  • Vitamin D-Binding Protein / genetics*

Substances

  • Vitamin D-Binding Protein
  • DNA
  • DNA Restriction Enzymes
  • Deoxyribonuclease BamHI