Molecular basis for two forms of the G protein that stimulates adenylate cyclase

J Biol Chem. 1986 Jul 25;261(21):9587-90.


Most cells contain two forms of the alpha subunit of the G protein (Gs) that stimulates adenylate cyclase; their apparent molecular weights are 45,000 and 52,000. Two cDNAs that correspond to distinct mRNAs for the alpha subunit of Gs have been cloned from a bovine adrenal library and sequenced. The sequences of the two cDNAs, designated pGs-l and pGs-S, are identical except for a single stretch of 46 nucleotides in the coding region, where four are altered and 42 are deleted in pGs-S. Expression of pGs-S and pGs-l in COS-m6 cells yields protein products with apparent molecular weights of 45,000 and 52,000, respectively, based on their mobility in sodium dodecyl sulfate-polyacrylamide gels. We conclude that pGs-S and pGs-l encode the 45- and 52-kDa forms of Gs alpha, respectively, and propose that the mRNAs encoding these proteins arise from a single gene by internal alternative RNA splicing.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenylyl Cyclases / genetics
  • Adenylyl Cyclases / metabolism*
  • Animals
  • Base Sequence
  • Cattle
  • DNA / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Endonucleases / metabolism
  • GTP-Binding Proteins / analysis*
  • GTP-Binding Proteins / pharmacology
  • Gene Expression Regulation
  • Immunosorbent Techniques
  • Molecular Weight
  • RNA Splicing
  • RNA, Messenger / metabolism
  • Single-Strand Specific DNA and RNA Endonucleases
  • Transcription, Genetic


  • RNA, Messenger
  • DNA
  • Endonucleases
  • Single-Strand Specific DNA and RNA Endonucleases
  • GTP-Binding Proteins
  • Adenylyl Cyclases

Associated data

  • GENBANK/M14014