Interaction Between XRCC1 Gene Polymorphisms and Obesity on Susceptibility to Papillary Thyroid Cancer in Chinese Han Population

Cell Physiol Biochem. 2018;49(2):638-644. doi: 10.1159/000493027. Epub 2018 Aug 30.

Abstract

Background/aims: To investigate the association of several single nucleotide polymorphisms (SNPs) within XRCC gene and additional gene- environment interaction with papillary thyroid cancer (PTC) risk.

Methods: Testing for Hardy-Weinberg equilibrium in controls was conducted using SNPstats (online software: http://bioinfo.iconcologia.net/SNPstats). Generalized multifactor dimensionality reduction (GMDR) was used to screen the best interaction combination among 5 SNPs within XRCC gene and obesity.

Results: Logistic regression analysis showed that the C allele of rs861539 and T allele of rs1799782 were associated with increased PTC risk Adjusted ORs (95%CI) were 1.65 (1.23-2.12) and 1.61 (1.20-2.04). However There was no relation of rs25489 Rs25487 and rs13181 with PTC. The cross-validation consistency and the testing accuracy for each of the models were determined by GMDR analysis. One two-locus model (rs1799782 and obesity) had a testing accuracy of 62.11% Which was significant at the p < 0.01 level. The D' value between rs1799782 and rs13181 within ERCC1 gene was more than 0.75 (0.825). So haplotype analysis was just conducted for rs1799782 and rs13181 using the SHEsis online haplotype analysis software. In all samples The haplotype C- A was observed most frequently in two groups With 49.46% and 55.79% in the PTC patients and controls Respectively. The results also indicated that haplotype T- C was significantly associated with increased PTC risk.

Conclusion: The C allele of rs861539 and T allele of rs1799782 Interaction between rs1799782 and obesity and haplotype T- C were all associated with increased PTC risk.

Keywords: Haplotype; Interaction; Obesity; Single nucleotide polymorphisms; Thyroid cancer; X-ray repair cross-complementing group.

MeSH terms

  • Aged
  • Alleles
  • Asian People / genetics*
  • Carcinoma, Papillary / diagnosis*
  • Carcinoma, Papillary / genetics
  • Case-Control Studies
  • China
  • Female
  • Genetic Predisposition to Disease
  • Genotype
  • Haplotypes
  • Humans
  • Logistic Models
  • Male
  • Middle Aged
  • Obesity / genetics*
  • Obesity / pathology
  • Polymorphism, Single Nucleotide
  • Risk
  • Thyroid Cancer, Papillary
  • Thyroid Neoplasms / diagnosis*
  • Thyroid Neoplasms / genetics
  • X-ray Repair Cross Complementing Protein 1 / genetics*

Substances

  • X-ray Repair Cross Complementing Protein 1
  • XRCC1 protein, human