CRISPR-Cas guides the future of genetic engineering

Science. 2018 Aug 31;361(6405):866-869. doi: 10.1126/science.aat5011.

Abstract

The diversity, modularity, and efficacy of CRISPR-Cas systems are driving a biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to manipulate the genomes of cultured cells, animals, and plants, accelerating the pace of fundamental research and enabling clinical and agricultural breakthroughs. We describe the basic mechanisms that set the CRISPR-Cas toolkit apart from other programmable gene-editing technologies, highlighting the diverse and naturally evolved systems now functionalized as biotechnologies. We discuss the rapidly evolving landscape of CRISPR-Cas applications, from gene editing to transcriptional regulation, imaging, and diagnostics. Continuing functional dissection and an expanding landscape of applications position CRISPR-Cas tools at the cutting edge of nucleic acid manipulation that is rewriting biology.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • CRISPR-Associated Protein 9
  • CRISPR-Cas Systems*
  • DNA / analysis
  • DNA / chemistry
  • DNA / genetics
  • Endonucleases / genetics
  • Endonucleases / metabolism
  • Gene Editing / methods*
  • Genetic Engineering / methods*
  • Genetic Engineering / trends*
  • Humans
  • Molecular Imaging
  • Plants / genetics
  • RNA / analysis
  • RNA / chemistry
  • RNA, Guide / genetics
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • RNA, Guide
  • RNA
  • DNA
  • CRISPR-Associated Protein 9
  • Cas9 endonuclease Streptococcus pyogenes
  • Endonucleases