Perspectives on Liquid Chromatography-High-Resolution Mass Spectrometry for Pesticide Screening in Foods

J Agric Food Chem. 2018 Sep 19;66(37):9573-9581. doi: 10.1021/acs.jafc.8b03468. Epub 2018 Sep 11.


This perspective discusses the use of liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) for multiresidue analysis of pesticides in foods and agricultural commodities. HRMS has the important distinction and advantage of mass-resolving power and, therefore, requires different concepts, experiments, and guidance for screening, identification, and quantitation of pesticides in complex food matrices over triple quadrupole mass spectrometry. HRMS approaches for pesticide screening, including full-scan experiments in conjunction with tandem mass spectrometry (MS/MS) experiments, are described. This approach results in the generation of chromatographic retention times and high-resolution mass spectra with accurate mass measurements that can be used to create compound databases. New data processing tools can create an efficient and optimized screening approach that can speed the analysis and identification of compounds, reduce the need for chemical standards, and harmonize pesticide analytical procedures.

Keywords: data dependent acquisition (DDA); data independent acquisition (DIA); liquid chromatography−high-resolution mass spectrometry (LC−HRMS); multiresidue pesticide analysis; non-target data acquisition target analysis (nDATA); pesticide screening.

Publication types

  • Review

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, Liquid
  • Food Contamination / analysis*
  • Pesticide Residues
  • Tandem Mass Spectrometry / instrumentation
  • Tandem Mass Spectrometry / methods*


  • Pesticide Residues