Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer

Org Biomol Chem. 2018 Nov 7;16(43):8056-8063. doi: 10.1039/c8ob01790e.

Abstract

Optical biosensing based on the activation of fluorescent reporters offers a powerful methodology for the real-time molecular interrogation of pathology. Here we report a first-in-class, bimodal fluorescent reporter strategy for the simultaneous and highly specific detection of two independent proteases (thrombin and matrix metalloproteases (MMPs)) pivotal in the fibroproliferative process surrounding lung cancer, based on a dual, multiplexing, peptide FRET system. This sophisticated synthetic smartprobe, with a molecular weight of 6 kDa, contains two independent fluorophores and quenchers that generate photonic signatures at two specific wavelengths upon activation by target enzymes within human lung cancer tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods*
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism
  • Humans
  • Lung Neoplasms / metabolism*
  • Lung Neoplasms / pathology
  • Matrix Metalloproteinases / metabolism
  • Neutrophils / metabolism
  • Plaque, Atherosclerotic / metabolism
  • Proteolysis*

Substances

  • Fluorescent Dyes
  • Matrix Metalloproteinases