XLSY: Extra-Large NMR Spectroscopy

Angew Chem Int Ed Engl. 2018 Oct 22;57(43):14043-14045. doi: 10.1002/anie.201806144. Epub 2018 Oct 1.


NMR studies of intrinsically disordered proteins and other complex biomolecular systems require spectra with the highest resolution and dimensionality. An efficient approach, extra-large NMR spectroscopy, is presented for experimental data collection, reconstruction, and handling of very large NMR spectra by a combination of the radial and non-uniform sampling, a new processing algorithm, and rigorous statistical validation. We demonstrate the first high-quality reconstruction of a full seven-dimensional HNCOCACONH and two five-dimensional HACACONH and HN(CA)CONH experiments for a representative intrinsically disordered protein α-synuclein. XLSY will significantly enhance the NMR toolbox in challenging biomolecular studies.

Keywords: NMR spectroscopy; XLSY; intrinsically disordered protein; non-uniform sampling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Intrinsically Disordered Proteins / chemistry
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Protein Conformation
  • alpha-Synuclein / chemistry


  • Intrinsically Disordered Proteins
  • alpha-Synuclein