Virus-mimetic polymer nanoparticles displaying hemagglutinin as an adjuvant-free influenza vaccine

Biomaterials. 2018 Nov:183:234-242. doi: 10.1016/j.biomaterials.2018.08.036. Epub 2018 Aug 23.

Abstract

The generation of virus-mimetic nanoparticles has received much attention in developing a new vaccine for overcoming the limitations of current vaccines. Thus, a method, encompassing most viral features for their size, hydrophobic domain and antigen display, would represent a meaningful direction for the vaccine development. In the present study, a polymer-templated protein nanoball with direction oriented hemagglutinin1 on its surface (H1-NB) was prepared as a new influenza vaccine, exhibiting most of the viral features. Moreover, the concentrations of antigen on the particle surface were controlled, and its effect on immunogenicity was estimated by in vivo studies. Finally, H1-NB efficiently promoted H1-specific immune activation and cross-protective activities, which consequently prevented H1N1 infections in mice.

Keywords: Antigen-specific immune activation; Influenza vaccine; Protein-polymer nanoparticle; Virus-mimicking; in situ method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomimetic Materials / chemistry
  • Biomimetic Materials / metabolism
  • Dendritic Cells / physiology
  • Hemagglutinins / chemistry*
  • Hemagglutinins / metabolism
  • Hemagglutinins, Viral / metabolism*
  • Humans
  • Influenza A Virus, H1N1 Subtype / immunology
  • Influenza Vaccines / chemistry*
  • Influenza Vaccines / immunology
  • Influenza Vaccines / metabolism
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Nanoparticles / chemistry*
  • Nanoparticles / metabolism
  • Orthomyxoviridae Infections / immunology
  • Orthomyxoviridae Infections / prevention & control
  • Particle Size
  • Polymers / chemistry*
  • Spleen / cytology

Substances

  • Hemagglutinins
  • Hemagglutinins, Viral
  • Influenza Vaccines
  • Polymers