A routine method for the establishment of permanent growing lymphoblastoid cell lines

Hum Genet. 1986 Aug;73(4):320-6. doi: 10.1007/BF00279094.


Permanent lymphoblastoid cell lines are of great practical value in human clinical and experimental genetics. A detailed protocol for routine use is given for the establishment of lymphoblastoid lines from peripheral blood using Epstein-Barr virus and the immunosuppressivum Cyclosporin A. In addition, the biologic basis of this transformation system is briefly summarized.

MeSH terms

  • Antigens, Viral / analysis
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / microbiology
  • B-Lymphocytes / ultrastructure
  • Cell Division / drug effects
  • Cell Line*
  • Cell Transformation, Viral*
  • Chromosome Aberrations*
  • Cyclosporins / pharmacology
  • Epstein-Barr Virus Nuclear Antigens
  • Ethidium / pharmacology
  • Herpesvirus 4, Human / immunology
  • Humans
  • Karyotyping


  • Antigens, Viral
  • Cyclosporins
  • Epstein-Barr Virus Nuclear Antigens
  • Ethidium