One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation

J Exp Med. 2018 Oct 1;215(10):2686-2695. doi: 10.1084/jem.20172064. Epub 2018 Sep 4.

Abstract

We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • Germinal Center / cytology
  • Germinal Center / immunology*
  • Immunoglobulin Class Switching*
  • Immunoglobulin Heavy Chains* / genetics
  • Immunoglobulin Heavy Chains* / immunology
  • Immunoglobulin kappa-Chains* / genetics
  • Immunoglobulin kappa-Chains* / immunology
  • Mice
  • Mice, Transgenic
  • Receptors, Antigen, B-Cell* / genetics
  • Receptors, Antigen, B-Cell* / immunology

Substances

  • Immunoglobulin Heavy Chains
  • Immunoglobulin kappa-Chains
  • Receptors, Antigen, B-Cell