The Three Members of the Arabidopsis Glycosyltransferase Family 92 are Functional β-1,4-Galactan Synthases

Plant Cell Physiol. 2018 Dec 1;59(12):2624-2636. doi: 10.1093/pcp/pcy180.

Abstract

Pectin is a major component of primary cell walls and performs a plethora of functions crucial for plant growth, development and plant-defense responses. Despite the importance of pectic polysaccharides their biosynthesis is poorly understood. Several genes have been implicated in pectin biosynthesis by mutant analysis, but biochemical activity has been shown for very few. We used reverse genetics and biochemical analysis to study members of Glycosyltransferase Family 92 (GT92) in Arabidopsis thaliana. Biochemical analysis gave detailed insight into the properties of GALS1 (Galactan synthase 1) and showed galactan synthase activity of GALS2 and GALS3. All proteins are responsible for adding galactose onto existing galactose residues attached to the rhamnogalacturonan-I (RG-I) backbone. Significant GALS activity was observed with galactopentaose as acceptor but longer acceptors are favored. Overexpression of the GALS proteins in Arabidopsis resulted in accumulation of unbranched β-1, 4-galactan. Plants in which all three genes were inactivated had no detectable β-1, 4-galactan, and surprisingly these plants exhibited no obvious developmental phenotypes under standard growth conditions. RG-I in the triple mutants retained branching indicating that the initial Gal substitutions on the RG-I backbone are added by enzymes different from GALS.

MeSH terms

  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Arabidopsis Proteins / metabolism*
  • Cell Wall / metabolism
  • Galactans / metabolism*
  • Genes, Plant
  • Glycosyltransferases / metabolism*
  • Golgi Apparatus / metabolism
  • Nicotiana / metabolism
  • Plant Leaves / metabolism
  • Recombinant Proteins / isolation & purification
  • Subcellular Fractions / metabolism
  • Substrate Specificity

Substances

  • Arabidopsis Proteins
  • Galactans
  • Recombinant Proteins
  • Glycosyltransferases