A DNA binding protein specific for an origin of replication of herpes simplex virus type 1

Proc Natl Acad Sci U S A. 1986 Sep;83(17):6322-6. doi: 10.1073/pnas.83.17.6322.


We have identified a protein that binds specifically to an origin of replication (oris) of the herpes simplex virus type 1 genome. The oris binding protein, detectable only in nuclear extracts of infected cells, shows the same time course of appearance as the herpesvirus-induced DNA polymerase and the DNA binding protein ICP8. The partially purified oris binding protein generates a DNase I "footprint" that spans 18- of the 90-base-pair minimal oris sequence. The oris binding protein may, therefore, be analogous to other origin-specific binding proteins that are required for the initiation of viral and chromosomal DNA replication.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Binding Sites
  • DNA Replication*
  • DNA-Binding Proteins / genetics*
  • Genes, Viral
  • Simplexvirus / genetics*
  • Time Factors
  • Viral Proteins / genetics
  • Virus Replication


  • DNA-Binding Proteins
  • Viral Proteins

Associated data

  • GENBANK/M13830