Immuno-affinity Amperometric Detection of Bacterial Infections

Angew Chem Int Ed Engl. 2018 Nov 5;57(45):14942-14946. doi: 10.1002/anie.201808666. Epub 2018 Oct 9.

Abstract

A combination of an immuno-affinity enrichment strategy and sensitive amperometric read-out was implemented in a point-of-care platform intended for bacterial infection analysis. Bacterial cells, selectively captured and enriched from complex matrices through immuno-affinity, were detected by amperometric monitoring of the redox state of metabolic activity indicators, providing species identification and viable-cell quantification. The method was successfully employed for the diagnosis of bacterial infections including antimicrobial susceptibility testing with only several hours of total working time.

Keywords: bacterial infections; biosensors; electrochemistry; metabolic activity; point-of-care testing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Immobilized / chemistry*
  • Bacteria / isolation & purification*
  • Bacterial Infections / diagnosis*
  • Biosensing Techniques / instrumentation*
  • Electrochemical Techniques / instrumentation*
  • Equipment Design
  • Humans
  • Immunomagnetic Separation / instrumentation*
  • Lab-On-A-Chip Devices
  • Point-of-Care Systems*

Substances

  • Antibodies, Immobilized