Conservation of the c-myc coding sequence in transduced feline v-myc genes

Virology. 1986 Oct 15;154(1):121-34. doi: 10.1016/0042-6822(86)90435-6.

Abstract

We have cloned the normal feline c-myc locus and determined the nucleotide sequence of all three exons. The feline c-myc gene shows close homology to other mammalian c-myc genes, particularly human c-myc. The feline and human sequences are colinear within the open reading frame for the putative c-myc product but show insertions and deletions relative to each other outside this domain. We have also analyzed a cloned FeLV provirus, CT4, which contains the host-derived myc gene. In this provirus the v-myc sequences are located at the 3' end of the pol gene, replacing pol and env sequences. Nucleotide sequence analysis of CT4 shows an open reading frame for a v-myc gene product which may be expressed without fusion to any viral protein sequences. This contrasts with another FeLV v-myc (LC), in which myc and gag sequences were found to be fused. Unlike previously identified avian v-myc genes, the feline v-myc genes contain exon 1-derived sequences, but these have been truncated or internally deleted. The FeLV CT4 v-myc sequence shows very few coding changes relative to c-myc and the FeLV LC v-myc coding sequence is unchanged relative to c-myc apart from fusion to gag. These results are discussed in relation to the mechanism of transduction and activation of myc by FeLV.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cloning, Molecular
  • Leukemia Virus, Feline / genetics*
  • Oncogenes*
  • Proto-Oncogenes*
  • Sequence Homology, Nucleic Acid
  • Transduction, Genetic
  • Viral Proteins / genetics

Substances

  • Viral Proteins

Associated data

  • GENBANK/M14889
  • GENBANK/M15204
  • GENBANK/M22726
  • GENBANK/M22727
  • GENBANK/M22728