Quantitation of Intact Proteins in Human Plasma Using Top-Down Parallel Reaction Monitoring-MS

Anal Chem. 2018 Sep 18;90(18):10650-10653. doi: 10.1021/acs.analchem.8b02699. Epub 2018 Sep 7.

Abstract

Direct quantitation of proteins in complex biological matrices by mass spectrometry remains a challenge. Here, we describe a novel top-down parallel reaction monitoring-mass spectrometry (PRM-MS) assay, enabled by microflow LC-nanospray MS using a silicon microfluidic LC-MS chip. We demonstrated direct analysis of intact proteins such as somatropin in human plasma, achieving sensitivity (0.1-1.0 fmole) and speed (1-5 min) on par with enzyme-linked immunosorbent assay (ELISA).

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Blood Proteins / analysis*
  • Chromatography, Liquid / methods
  • Enzyme-Linked Immunosorbent Assay
  • HeLa Cells
  • Humans
  • Lab-On-A-Chip Devices
  • Limit of Detection
  • Reproducibility of Results
  • Tandem Mass Spectrometry / methods*

Substances

  • Blood Proteins