Dihydromyricetin from ampelopsis grossedentata protects against vascular neointimal formation via induction of TR3

Eur J Pharmacol. 2018 Nov 5;838:23-31. doi: 10.1016/j.ejphar.2018.09.002. Epub 2018 Sep 5.


Vine tea has been used as a medicinal herb in traditional Chinese medicine for hundreds of years. As the most abundant ingredient in vine tea, Dihydromyricetin (DHM) has been reported to exert anti-inflammatory, antioxidant, and anti-cardiovascular disease. However, the role of DHM in injury-induced neointimal formation remains poorly characterized. We determined the effects of DHM on ligation-induced carotid artery neointimal formation. We found that ligation-induced carotid artery neointimal formation could be significantly attenuated by DHM treatment. We provide evidence that DHM increases orphan nuclear receptor TR3 expression in smooth muscle cell (SMC) and carotid artery. Moreover, overexpression and loss-of-function strategies of TR3 were done to overexpression and knockdown of TR3, and demonstrate that DHM promotes SMC differentiation, however, inhibits SMC proliferation and migration, via regulating expression of TR3. Collectively, we reveal that DHM may be a therapeutic agent for the treatment of injury-induced vascular diseases.

Keywords: Dihydromyricetin; Neointimal formation; Smooth muscle cell; TR3.

MeSH terms

  • Ampelopsis / chemistry*
  • Animals
  • Carotid Arteries / drug effects
  • Carotid Arteries / pathology
  • Carotid Stenosis / drug therapy*
  • Carotid Stenosis / etiology
  • Carotid Stenosis / pathology
  • Cell Line
  • Cell Proliferation / drug effects
  • Disease Models, Animal
  • Flavonols / pharmacology*
  • Flavonols / therapeutic use
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Muscle, Smooth, Vascular / cytology
  • Muscle, Smooth, Vascular / drug effects
  • Muscle, Smooth, Vascular / pathology
  • Myocytes, Smooth Muscle / drug effects
  • Myocytes, Smooth Muscle / metabolism
  • Myocytes, Smooth Muscle / pathology
  • Neointima / drug therapy*
  • Neointima / etiology
  • Neointima / pathology
  • Nuclear Receptor Subfamily 4, Group A, Member 1 / metabolism*
  • Rats


  • Flavonols
  • Nuclear Receptor Subfamily 4, Group A, Member 1
  • dihydromyricetin