Purification and characterization of a novel protease-resistant GH27 α-galactosidase from Hericium erinaceus

Int J Biol Macromol. 2018 Dec;120(Pt B):2165-2174. doi: 10.1016/j.ijbiomac.2018.09.021. Epub 2018 Sep 5.


A novel 57-kDa acidic α-galactosidase designated as HEG has been purified from the dry fruiting bodies of Hericium erinaceus. The isolation protocol involved ion-exchange chromatography and gel filtration on a Superdex75 column. The purification fold and specific activity were 1251 and 46 units/mg, respectively. A BLAST search of internal peptide sequences obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis suggested that the enzyme belonged to the GH27 family. The activity of the enzyme reached its maximum at a pH of 6.0 or at 60 °C. The enzyme was stable within an acidic pH range of 2.2-7.0 and in a narrow temperature range. The enzyme was strongly inhibited by Zn2+, Fe3+, Ag+ ions and SDS. The Lineweaver-Burk plot suggested that the mode of inhibition by galactose and melibiose were of a mixed type. N-bromosuccinimide drastically decreased the activity of the enzyme, whereas diethylpyrocarbonate and carbodiimide strengthened the activity slightly. Moreover, the isolated enzyme displayed remarkable resistance to acid proteases, neutral proteases and pepsin. The enzyme could also hydrolyse oligosaccharides and polysaccharides. In addition, acidic protease promoted the hydrolysis of RFOs by HEG. The Km values of the enzyme towards pNPGal, raffinose and stachyose were 0.36 mM, 40.07 mM and 54.71 mM, respectively. These favourable properties increase the potential of the enzyme in the food industry and animal feed applications.

Keywords: GH27; Hericium erinaceus; Raffinose-family oligosaccharide; α-Galactosidase.

MeSH terms

  • Amino Acid Sequence
  • Basidiomycota / enzymology*
  • Enzyme Inhibitors / pharmacology
  • Hydrolysis
  • Kinetics
  • Molecular Weight
  • Peptide Hydrolases / metabolism*
  • Raffinose / metabolism
  • Substrate Specificity
  • alpha-Galactosidase / antagonists & inhibitors
  • alpha-Galactosidase / chemistry
  • alpha-Galactosidase / isolation & purification*
  • alpha-Galactosidase / metabolism*


  • Enzyme Inhibitors
  • alpha-Galactosidase
  • Peptide Hydrolases
  • Raffinose