Extracellular Matrix Remodeling Regulates Glucose Metabolism through TXNIP Destabilization

Cell. 2018 Sep 20;175(1):117-132.e21. doi: 10.1016/j.cell.2018.08.017. Epub 2018 Sep 6.


The metabolic state of a cell is influenced by cell-extrinsic factors, including nutrient availability and growth factor signaling. Here, we present extracellular matrix (ECM) remodeling as another fundamental node of cell-extrinsic metabolic regulation. Unbiased analysis of glycolytic drivers identified the hyaluronan-mediated motility receptor as being among the most highly correlated with glycolysis in cancer. Confirming a mechanistic link between the ECM component hyaluronan and metabolism, treatment of cells and xenografts with hyaluronidase triggers a robust increase in glycolysis. This is largely achieved through rapid receptor tyrosine kinase-mediated induction of the mRNA decay factor ZFP36, which targets TXNIP transcripts for degradation. Because TXNIP promotes internalization of the glucose transporter GLUT1, its acute decline enriches GLUT1 at the plasma membrane. Functionally, induction of glycolysis by hyaluronidase is required for concomitant acceleration of cell migration. This interconnection between ECM remodeling and metabolism is exhibited in dynamic tissue states, including tumorigenesis and embryogenesis.

Keywords: GLUT1 trafficking; TXNIP; ZFP36; cell biology; cell migration; extracellular matrix; glucose metabolism; hyaluronidase; mRNA degradation; receptor tyrosine kinase signaling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Metabolism / physiology
  • Carrier Proteins / metabolism
  • Carrier Proteins / physiology*
  • Cell Line, Tumor
  • Extracellular Matrix / metabolism*
  • Extracellular Matrix / physiology*
  • Glucose / metabolism
  • Glucose Transporter Type 1
  • Glycolysis / physiology
  • Humans
  • Hyaluronic Acid / physiology
  • Hyaluronoglucosaminidase / pharmacology
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Signal Transduction
  • Tristetraprolin / metabolism
  • Tristetraprolin / physiology


  • Carrier Proteins
  • Glucose Transporter Type 1
  • Intercellular Signaling Peptides and Proteins
  • SLC2A1 protein, human
  • TXNIP protein, human
  • Tristetraprolin
  • ZFP36 protein, human
  • Hyaluronic Acid
  • Hyaluronoglucosaminidase
  • Glucose