Abstract
We investigated the relationship between Neutrophil-to-lymphocyte ratio (NLR) and the quantities of neutrophil elastase, CD3, Foxp3, and CD204 in tumor-infiltrating cells and circulating cytokines (IL-2, -6, -8, 17a, and TGF-β) in muscle-invasive bladder cancer. IL-6 and IL-8 levels showed a significant correlation with NLR in blood and Foxp3+ cells around the tumor. After co-culture of peripheral blood cells with bladder cancer cell lines, the induction of regulatory T cell (Treg) was higher in T24 whose IL-6 and IL-8 levels were higher. High NLR correlates with increased IL-6 and IL-8 and Treg expression.
Keywords:
Foxp3; IL-6; IL-8; NLR; muscle-invasive bladder cancer.
MeSH terms
-
Aged
-
CD4 Lymphocyte Count
-
Cell Line, Tumor
-
Coculture Techniques
-
Cytokines / blood
-
Cytokines / immunology*
-
Cytokines / urine
-
Enzyme-Linked Immunosorbent Assay
-
Female
-
Forkhead Transcription Factors / blood
-
Forkhead Transcription Factors / immunology
-
Humans
-
Immunophenotyping
-
Lymphocytes, Tumor-Infiltrating / immunology*
-
Lymphocytes, Tumor-Infiltrating / metabolism
-
Male
-
Middle Aged
-
Muscle, Smooth / immunology*
-
Muscle, Smooth / metabolism
-
Muscle, Smooth / pathology
-
Neoplasm Invasiveness
-
Neutrophils / immunology*
-
Neutrophils / metabolism
-
Phenotype
-
Prognosis
-
Retrospective Studies
-
T-Lymphocytes, Regulatory / immunology*
-
T-Lymphocytes, Regulatory / metabolism
-
Tumor Microenvironment*
-
Urinary Bladder Neoplasms / blood
-
Urinary Bladder Neoplasms / immunology*
-
Urinary Bladder Neoplasms / pathology
-
Urinary Bladder Neoplasms / urine
Substances
-
Cytokines
-
FOXP3 protein, human
-
Forkhead Transcription Factors