hCD46 receptor is not required for measles vaccine Schwarz strain replication in vivo: Type-I IFN is the species barrier in mice

Virology. 2018 Nov;524:151-159. doi: 10.1016/j.virol.2018.08.014. Epub 2018 Sep 7.


Measles virus has been successfully attenuated on chicken embryo cells to obtain a highly efficient and safe live attenuated vaccine, administered thus far to billions of children. Measles virus attenuation has long been described to involve a modification of cellular tropism with the use of human CD46 ubiquitous receptor. Nevertheless, the use of this receptor in vivo is not obvious. In this study we use four different mouse models to decipher the respective part of hCD46 receptor and type-I interferon response in measles host restriction. We observed that only type-I interferon restricts viral replication of attenuated MV Schwarz strain in mice, independently of the presence of hCD46 receptor. By comparing measles virus immunogenicity in the different models, we confirmed that there was no impact on the absence of this receptor on the immune response. Therefore, we propose to simplify the mouse model.

Keywords: Cellular tropism; Host restriction; Measles live attenuated vaccine; Mouse model; Type-I interferon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chlorocebus aethiops
  • Humans
  • Interferon Type I / immunology*
  • Measles / prevention & control
  • Measles / virology*
  • Measles Vaccine / immunology*
  • Measles virus / immunology
  • Measles virus / physiology*
  • Membrane Cofactor Protein / genetics
  • Membrane Cofactor Protein / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Species Specificity
  • Vaccines, Attenuated / immunology
  • Vero Cells
  • Virus Replication*


  • CD46 protein, human
  • Interferon Type I
  • Measles Vaccine
  • Membrane Cofactor Protein
  • Vaccines, Attenuated