Streptocyclinones A and B ameliorate Alzheimer's disease pathological processes in vitro

Neuropharmacology. 2018 Oct;141:283-295. doi: 10.1016/j.neuropharm.2018.09.008. Epub 2018 Sep 8.

Abstract

Alzheimer's disease (AD) is a pathology characterized by the abnormal accumulation of amyloid-beta (Aβ) and hyperphosphorylated tau. Oxidative stress and neuroinflammation are also strongly related to this disease. The ability of two new glycosylated angucyclinones, streptocyclinones A and B (1 and 2), isolated from Streptomyces sp to improve AD hallmarks was evaluated. Compounds were able to protect SH-SY5Y neuroblastoma cells from H2O2-induced oxidative injury by activating the nuclear factor E2-related factor (Nrf2). Their capacity to modulate neuroinflammation was tested in lipopolysaccharide-activated BV2 microglial cells. Compounds reduced the release of pro-inflammatory factors, inhibited the activation of NFκB and mitogen activated kinases (MAPK), and induced the translocation of Nrf2 to the nucleus of microglial cells. A trans-well co-culture was established to determine the effect of microglia treated with streptocyclinones on the survival of SH-SY5Y cells. The cell viability of neuroblastoma cells increased when the compounds were added to BV2 cells. SH-SY5Y-TMHT441 cells were used to determine the effect of compounds on tau phosphorylation. Both compounds reduced tau hyperphophorylation by targeting MAPK kinases. Moreover, streptocyclinone B (2) was able to inhibit the activity of β-secretase 1 and decrease the release of reactive oxygen species in BV2 cells stimulated with Aβ. With the same co-culture trans-well system, the treatment of Aβ-stimulated microglia with compound 2 augmented the viability of SH-SY5Y-TMHT441 cells. The results presented in this work provide evidences of the multitarget activities displayed by these new Streptomyces compounds, making them good candidates for further studies in the treatment of AD.

Keywords: Alzheimer disease; Neuroinflammation; Nrf2; Oxidative stress; Streptomyces; p38.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / drug therapy*
  • Alzheimer Disease / pathology*
  • Amyloid Precursor Protein Secretases / antagonists & inhibitors
  • Animals
  • Anthraquinones / chemistry
  • Anthraquinones / isolation & purification
  • Anthraquinones / pharmacology
  • Anthraquinones / therapeutic use*
  • Cell Survival / drug effects
  • Cells, Cultured
  • Coculture Techniques
  • Humans
  • Hydrogen Peroxide
  • Inflammation Mediators / metabolism
  • Lipopolysaccharides
  • Mice
  • Mitogen-Activated Protein Kinases / metabolism
  • NF-E2-Related Factor 2 / metabolism
  • NF-kappa B / metabolism
  • Neuroprotective Agents / pharmacology
  • Phosphorylation / drug effects
  • Reactive Oxygen Species / metabolism
  • tau Proteins / metabolism

Substances

  • Anthraquinones
  • Inflammation Mediators
  • Lipopolysaccharides
  • NF-E2-Related Factor 2
  • NF-kappa B
  • Neuroprotective Agents
  • Reactive Oxygen Species
  • angucyclinone
  • tau Proteins
  • Hydrogen Peroxide
  • Mitogen-Activated Protein Kinases
  • Amyloid Precursor Protein Secretases