Characterization of a new type of human papillomavirus (HPV) related to HPV5 from a case of actinic keratosis

Virology. 1986 Oct 30;154(2):389-94. doi: 10.1016/0042-6822(86)90464-2.


Human papillomavirus (HPV) DNA sequences, related to the genomes of HPVs associated with epidermodysplasia verruciformis (EV), were detected in DNA samples extracted from biopsied lesions in 2 of 24 cases of actinic keratosis found in the general population. An HPV DNA was molecularly cloned from one of these samples. Blot hybridization experiments, performed under stringent conditions, revealed a significant cross-hybridization only between this HPV DNA and the DNAs of HPV5 and of the HPV5-related types. The extent of homology between them ranged from 7 to 30%, as evaluated by hybridization in liquid phase at saturation followed by nuclease S1 analysis. This showed that the cloned HPV represented a new type, tentatively named HPV36. HPV36 was not found in the other 22 cases of actinic keratosis, but was detected in scrapings of benign lesions of 7 of 18 (39%) EV patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Cloning, Molecular
  • DNA, Viral / analysis*
  • Female
  • Genes, Viral
  • Humans
  • Keratosis / microbiology*
  • Male
  • Middle Aged
  • Nucleic Acid Hybridization
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Sequence Homology, Nucleic Acid


  • DNA, Viral