Replacement of the deletion in the genome (0.762-0.789 mu) of avirulent HSV-1 HFEM using cloned MluI DNA fragment (0.7615-0.796 mu) of virulent HSV-1 F leads to generation of virulent intratypic recombinant

Virus Res. 1986 Aug;5(2-3):157-75. doi: 10.1016/0168-1702(86)90015-8.

Abstract

The HFEM strain of HSV-1 is apathogenic for the tree shrew by the intraperitoneal (i.p.) route because of a deletion in the genome coordinates 0.762-0.789. Insertion of the MluI DNA fragment (coordinates 0.7615-0.789) cloned from HSV-1 strain F, which is pathogenic for the tree shrew, restored the i.p. pathogenicity to strain HFEM. The recombinant designated R-M1-C1 was highly pathogenic for the tree shrew, but slightly virulent for inbred mouse strain A. It thus appears that the viral DNA sequence involved in the i.p. pathogenicity of HSV-1 is located within the genome coordinates 0.761-0.796. This sequence is recognized differently by the cellular elements involved in HSV-1 infection in the tree shrew and the mouse.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Chromosome Deletion*
  • Cloning, Molecular*
  • DNA / analysis*
  • DNA Restriction Enzymes
  • Female
  • Genes, Viral*
  • Male
  • Mice
  • Nucleic Acid Hybridization
  • Simplexvirus / genetics*
  • Simplexvirus / isolation & purification
  • Simplexvirus / pathogenicity
  • Transfection
  • Tupaia
  • Virulence

Substances

  • DNA
  • DNA Restriction Enzymes