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. 2018 Aug 15;10(8):2350-2361.
eCollection 2018.

Serum NOX2 as a New Biomarker Candidate for HBV-related Disorders

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Free PMC article

Serum NOX2 as a New Biomarker Candidate for HBV-related Disorders

Yuan Xiong et al. Am J Transl Res. .
Free PMC article

Abstract

Growing evidence supports the notion that serum NAPDH oxidase 2 (NOX2) is an important regulator that contributes to the initiation and progression of various types of diseases. However, so far, it remains elusive about the relationship between levels of serum NOX2 and HBV-related diseases. The overall purpose of the study is to get a better insight into whether or not serum NOX2 is involved in HBV-related disorders. Serum levels of NOX2, from 105 patients with chronic hepatitis B, 58 patients with HBV-related cirrhosis, 48 patients with HBV-related hepatocellular carcinoma and 104 healthy individuals, were measured with sandwich ELISA kits that we developed. In this study, we found that NOX2 values were significantly higher in patients compared to healthy control (P < 0.01) and that the levels of serum NOX2 were significantly correlated with the serum levels of superoxide dismutase (SOD), interleukin-6 (IL-6), interferon-stimulated gene 15 (ISG15), alkaline phosphatase (ALP) and gamma glutamyl transpeptidase (GGT) in chronic hepatitis B, cirrhosis and hepatocellular carcinoma patients. Interestingly, we found that a significant positive correlation between NOX2 and HBV viral load only in patients with chronic hepatitis B and cirrhosis. Therefore, Serum NOX2 levels maybe an important indicator of the pathogenesis of progression of HBV-related disorders.

Keywords: Hepatitis B virus; NOX2; ROS; chronic hepatitis B; cirrhosis; hepatocellular carcinoma.

Conflict of interest statement

None.

Figures

Figure 1
Figure 1
Serum NOX2 concentrations in subjects. A. The scatter dot plot shows the serum levels of NOX2 in healthy individuals and patients with CHB, cirrhosis and HCC patients. B. The scatter dot plot demonstrates the serum levels of NOX2 in patients with HBV-related cirrhosis and alcoholic liver cirrhosis patients. C. The scatter dot plot shows the serum levels of NOX2 in patients with HBV-related HCC and non-HBV-related HCC. The middle horizontal line in the scatter dot plot represents the mean. And the horizontal lines below and above the mean line represent respectively the mean minus and plus one standard deviation. Statistical significance (P) is indicated.
Figure 2
Figure 2
Serum NOX2 concentrations in HBeAg-positive and HBeAg-negative patients. A. The scatter dot plot shows the serum levels of NOX2 in patients with HBeAg-negative CHB and HBeAg-positive CHB. B. The scatter dot plot demonstrates the serum levels of NOX2 in patients with HBeAg-negative cirrhosis and HBeAg-positive cirrhosis. C. The scatter dot plot shows the serum levels of NOX2 in patients with HBeAg-negative HCC and HBeAg-positive HCC. The middle horizontal line in the scatter dot plot represents the mean. And the horizontal lines below and above the mean line represent respectively the mean minus and plus one standard deviation. Statistical significance (P) is indicated.
Figure 3
Figure 3
Correlation between NOX2 and HBV viral load in patients. A. Serum NOX2 levels are positively correlated with HBV viral load in patients with CHB. B. Serum NOX2 concentrations are moderately positively correlated with HBV viral load in patients with HBV-related cirrhosis. C. There is no correlation between NOX2 and HBV viral load in patients with HCC. Correlation coefficient (r) and statistical significance (P) are indicated.
Figure 4
Figure 4
Correlation between NOX2 and SOD in patients. A. The scatter dot plot shows the serum levels of SOD in CHB, cirrhosis and HCC patients. The middle horizontal line in the scatter dot plot represents the mean. And the horizontal lines below and above the mean line represent respectively the mean minus and plus one standard deviation. B. Serum NOX2 values are negatively correlated with SOD levels in patients with CHB. C. Serum NOX2 concentrations are negatively correlated with SOD levels in cirrhosis patients. D. Serum NOX2 values are negatively correlated with SOD levels in HCC patients. Correlation coefficient (r) and statistical significance (P) are indicated.
Figure 5
Figure 5
Correlation between NOX2 and IL-6 in patients. A. The scatter dot plot shows the serum levels of IL-6 in CHB, cirrhosis and HCC patients. The middle horizontal line in the scatter dot plot represents the mean. And the horizontal lines below and above the mean line represent respectively the mean minus and plus one standard deviation. B. Serum NOX2 values are positively correlated with IL-6 levels in patients in CHB patients. C. Serum NOX2 values are positively correlated with IL-6 levels in patients in cirrhosis patients. D. Serum NOX2 values are positively correlated with IL-6 levels in patients in HCC patients. Correlation coefficient (r) and statistical significance (P) are indicated.
Figure 6
Figure 6
Correlation between NOX2 and ISG15 in patients. A. The scatter dot plot shows the serum levels of SG15 in CHB, cirrhosis and HCC pa-tients. The middle horizontal line in the scatter dot plot represents the mean. And the horizontal lines below and above the mean line represent respectively the mean minus and plus one standard deviation. B. Serum NOX2 values are positively correlated with ISG15 levels in patients in CHB patients. C. Serum NOX2 values are positively correlated with ISG15 levels in patients in cirrhosis patients. D. Serum NOX2 values are positively correlated with IL-6 levels in patients in HCC patients. Correlation coefficient (r) and statistical significance (P) are indicated.
Figure 7
Figure 7
Correlation between NOX2 and ALP in patients. A. Serum NOX2 values are positively correlated with ALP levels in CHB patients. B. Serum NOX2 values are positively correlated with ALP levels in cirrhosis patients. C. Serum NOX2 values are positively correlated with ALP levels in HCC patients. Correlation coefficient (r) and statistical significance (P) are indicated.
Figure 8
Figure 8
Correlation between NOX2 and GGT in patients. A. Serum NOX2 values are positively correlated with GGT levels in CHB patients. B. Serum NOX2 values are positively correlated with GGT levels in cirrhosis patients. C. Serum NOX2 values are positively correlated with GGT levels in HCC patients. Correlation coefficient (r) and statistical significance (P) are indicated.

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