Genome sequence of Rhizomucor pusillus FCH 5.7, a thermophilic zygomycete involved in plant biomass degradation harbouring putative GH9 endoglucanases

Silvia Hüttner; Zoraide Granchi; Thanh Thuy Nguyen; Sake van Pelt; Johan Larsbrink; Vu Nguyen Thanh; Lisbeth Olsson

doi:10.1016/j.btre.2018.e00279

Genome sequence of Rhizomucor pusillus FCH 5.7, a thermophilic zygomycete involved in plant biomass degradation harbouring putative GH9 endoglucanases

Biotechnol Rep (Amst). 2018 Aug 31:20:e00279. doi: 10.1016/j.btre.2018.e00279. eCollection 2018 Dec.

Authors

Silvia Hüttner^{1

2}, Zoraide Granchi³, Thanh Thuy Nguyen⁴, Sake van Pelt³, Johan Larsbrink^{1

2}, Vu Nguyen Thanh⁴, Lisbeth Olsson^{1

2}

Affiliations

¹ Department of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
² Wallenberg Wood Science Center, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
³ GenomeScan B.V., Plesmanlaan 1/D, 2333 BZ Leiden, The Netherlands.
⁴ Centre for Industrial Microbiology, Food Industries Research Institute, Thanh Xuan, Ha Noi, Viet Nam.

Abstract

We report here the annotated draft genome sequence of the thermophilic zygomycete Rhizomucor pusillus strain FCH 5.7, isolated from compost soil in Vietnam. The genome assembly contains 25.59 Mb with an overall GC content of 44.95%, and comprises 10,898 protein coding genes. Genes encoding putative cellulose-, xylan- and chitin-degrading proteins were identified, including two putative endoglucanases (EC 3.2.1.4) from glycoside hydrolase family 9, which have so far been mostly assigned to bacteria and plants.

Keywords: CAZyme; Carbohydrate-active enzymes; Endoglucanase; GH9; R. miehei; Thermophilic.