1,25-Dihydroxyvitamin D3 Inhibits the Clonogenic Growth of Transformed Cells via Its Receptor

Biochem Biophys Res Commun. 1986 Aug 29;139(1):136-43. doi: 10.1016/s0006-291x(86)80090-0.

Abstract

Anchorage-independent growth in soft agar is a unique property of transformed cells which is known to be correlated with tumorigenicity. We report here that 1,25-dihydroxyvitamin D3 suppresses colony formation by a number of cultured cancer cell lines in soft agar in a dose dependent manner with an ID50 of 5-7 X 10(-10) M. This effect is also achieved with analogues of 1,25-dihydroxyvitamin D3 in accordance with their binding affinity for the hormone's receptor. Only cells with 1,25-dihydroxyvitamin D3 receptor protein are inhibited in their colony formation by vitamin D analogs indicating that the hormone receptor complex may be integrally involved in the in vitro suppression of the anchorage-independent phenotype.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Calcitriol / pharmacology*
  • Cell Line
  • DNA / metabolism
  • Neoplastic Stem Cells / drug effects*
  • Receptors, Calcitriol
  • Receptors, Steroid / analysis
  • Receptors, Steroid / physiology*
  • Vitamin D / pharmacology

Substances

  • Antineoplastic Agents
  • Receptors, Calcitriol
  • Receptors, Steroid
  • Vitamin D
  • DNA
  • Calcitriol