The role of phospholipids in the binding of antimycin to yeast Complex III

Biochim Biophys Acta. 1986 Nov 5;852(1):100-5. doi: 10.1016/0005-2728(86)90061-7.

Abstract

Treatment of yeast Complex III with hexane or repeated fractionation with ammonium sulfate-cholate abolishes the ability of antimycin to bind to the complex. Antimycin binding is partially restored by addition of phospholipids to the depleted complex; this action of phospholipids can be enhanced by including Q6 in the reconstitution mixture.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antimycin A / analogs & derivatives*
  • Antimycin A / metabolism
  • Cardiolipins / pharmacology
  • Electron Transport Complex III / metabolism*
  • Fluorescence
  • Phospholipids / pharmacology*
  • Ubiquinone / physiology

Substances

  • Cardiolipins
  • Phospholipids
  • antimycin
  • Ubiquinone
  • Antimycin A
  • Electron Transport Complex III