miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR

Onco Targets Ther. 2018 Aug 30;11:5293-5302. doi: 10.2147/OTT.S173416. eCollection 2018.


Background: The roles and related mechanism of miR-376a in breast cancer cell progression are unclear.

Methods: Kaplan-Meier plotter analysis was used to analyze the correlation between miR-376a and the overall survival (OS) of breast cancer patients. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to detect miR-376a level in breast cancer cells. Cell viability, transwell migration and invasion, and cell apoptosis were constructed to investigate the effects of miR-376a on breast cancer cells. Luciferase reporter and RNA immunoprecipitation (RIP) were used to explore the targeting of miR-376a on NRP-1.

Results: miR-376a expression was positively correlated with the overall survival of breast cancer patients, and significantly decreased in breast cancer cells. Functionally, miR-376a over-expression suppressed cell proliferation, migration and invasion, and promoted cells apoptosis. Additionally, miR-376a could directly target NRP-1 and exerted its effect through NRP-1.

Conclusion: miR-376a could suppress breast cancer cell progression via directly targeting NRP-1.

Keywords: NRP-1t; Wn/β-catenin migration; breast cancert; miR-376at.