Portable Infrared Isothermal PCR Platform for Multiple Sexually Transmitted Diseases Strand Detection

Antony R Warden; Wenjia Liu; Huixing Chen; Xianting Ding

doi:10.1021/acs.analchem.8b03507

Portable Infrared Isothermal PCR Platform for Multiple Sexually Transmitted Diseases Strand Detection

Anal Chem. 2018 Oct 16;90(20):11760-11763. doi: 10.1021/acs.analchem.8b03507. Epub 2018 Sep 24.

Authors

Antony R Warden¹, Wenjia Liu¹, Huixing Chen², Xianting Ding¹

Affiliations

¹ State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering , Shanghai Jiao Tong University , Shanghai 200030 , China.
² Department of Andrology and PFD, Center for Men's Health and Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine , Shanghai Jiao Tong University , Shanghai 200080 , China.

PMID: 30216046
DOI: 10.1021/acs.analchem.8b03507

Abstract

Rapid and portable PCR detection is essential for screening sexually transmitted infections regularly. We developed an infrared mediated RNA isothermal RT-PCR (IR-MERIT PCR) platform and its compatible multichamber microfluidic chip for simultaneous amplification and testing (SAT) detection. This microfluidic chip integrates RNA extraction, micropump, and multitarget detection function onto the same chip. By utilizing IR-light-emitting diode (LED) as heat source, this platform can fulfill isothermal amplification within 70 min.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fluorescence
Humans
Infrared Rays
Microfluidic Analytical Techniques
Polymerase Chain Reaction*
RNA, Ribosomal, 16S / analysis*
RNA, Ribosomal, 16S / genetics*
Sexually Transmitted Diseases / diagnosis*
Sexually Transmitted Diseases / microbiology
Sexually Transmitted Diseases / virology*

Substances

RNA, Ribosomal, 16S