Antiviral effects of recombinant tumour necrosis factor in vitro

Nature. 1986 Oct 30-Nov 5;323(6091):816-9. doi: 10.1038/323816a0.

Abstract

Tumour necrosis factor (TNF) was first described as a factor in the serum of mice injected with tubercle bacilli (BCG) and several days later with lipopolysaccharide (LPS). The gene encoding TNF has recently been cloned and pure recombinant human TNF is now available. TNF is known for its in vivo antitumour effect and in vitro cytotoxicity on certain transformed cell lines. Similarities in amino acid sequence and biological activity to lymphotoxin and cachectin have been reported, and very recently a growth-factor-like activity on diploid fibroblasts was observed. There is no similarity between these proteins and interferons (IFNs), which are also induced during in vivo induction of TNF. Here we describe the antiviral activity of pure recombinant human TNF in a typical in vitro antiviral assay which we discovered while investigating the possible role of TNF as an inducer of IFN.

MeSH terms

  • 2',5'-Oligoadenylate Synthetase / metabolism
  • Animals
  • Antiviral Agents / therapeutic use*
  • Cell Line
  • Encephalomyocarditis virus / drug effects
  • Enzyme Activation
  • Glycoproteins / therapeutic use*
  • Humans
  • In Vitro Techniques
  • Interferon Type I / pharmacology
  • Mice
  • Rats
  • Recombinant Proteins / therapeutic use*
  • Simplexvirus / drug effects
  • Time Factors
  • Tumor Necrosis Factor-alpha
  • Vesicular stomatitis Indiana virus / drug effects

Substances

  • Antiviral Agents
  • Glycoproteins
  • Interferon Type I
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • 2',5'-Oligoadenylate Synthetase