Label-free prediction of three-dimensional fluorescence images from transmitted-light microscopy

Nat Methods. 2018 Nov;15(11):917-920. doi: 10.1038/s41592-018-0111-2. Epub 2018 Sep 17.


Understanding cells as integrated systems is central to modern biology. Although fluorescence microscopy can resolve subcellular structure in living cells, it is expensive, is slow, and can damage cells. We present a label-free method for predicting three-dimensional fluorescence directly from transmitted-light images and demonstrate that it can be used to generate multi-structure, integrated images. The method can also predict immunofluorescence (IF) from electron micrograph (EM) inputs, extending the potential applications.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cells, Cultured
  • Cellular Structures / ultrastructure*
  • Fibrosarcoma / pathology
  • Fluorescent Antibody Technique*
  • HEK293 Cells
  • Humans
  • Image Processing, Computer-Assisted / methods*
  • Imaging, Three-Dimensional / methods*
  • Induced Pluripotent Stem Cells / cytology
  • Microscopy, Electron / methods*
  • Microscopy, Fluorescence / methods*