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. 2017 Jul 25;1(2):170-175.
doi: 10.1016/j.mayocpiqo.2017.06.001. eCollection 2017 Sep.

An Assessment of the AdenoPlus Point-of-Care Test for Diagnosing Adenoviral Conjunctivitis and Its Effect on Antibiotic Stewardship

Kelly K Holtz  1 , Kalie R Townsend  1   2 , Joseph W Furst  1 , Jane F Myers  1 , Matthew J Binnicker  3 , Stephanie M Quigg  1 , Julie A Maxson  1 , Mark J Espy  3
Affiliations

An Assessment of the AdenoPlus Point-of-Care Test for Diagnosing Adenoviral Conjunctivitis and Its Effect on Antibiotic Stewardship

Kelly K Holtz et al. Mayo Clin Proc Innov Qual Outcomes. .

Abstract

Objective: To determine the sensitivity and specificity of the AdenoPlus test compared with real-time polymerase chain reaction (PCR) and to determine whether there was a reduction in antibiotic prescriptions with the use of AdenoPlus compared with the previous year.

Patients and methods: A total of 125 patients with suspected infectious conjunctivitis were accrued from June 4, 2015, through September 27, 2015. Forty-six participants from the prospective cohort completed both AdenoPlus and PCR testing. Two hundred fifty age-matched individuals were in the retrospective cohort.

Results: There was a significant reduction in the percentage of patients who received an antibiotic ophthalmic prescription in the prospective cohort vs the retrospective cohort (32% vs 45%; χ2P=.01). AdenoPlus test sensitivity was 50% (5 of 10) and specificity was 92% (33 of 36) compared with real-time PCR testing.

Conclusion: The AdenoPlus test has high specificity for diagnosing adenoviral conjunctivitis but lower sensitivity than has been previously published. These data suggest that negative AdenoPlus results should be confirmed by real-time PCR owing to the low overall sensitivity of AdenoPlus observed.

Keywords: HIPAA, Health Insurance Portability and Accountability Act; PCR, polymerase chain reaction; POC, point-of-care.

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