Lentzea terrae sp. nov., isolated from soil and an emended description of Lentzea soli

Abstract

A novel actinobacterium, designated strain NEAU-LZS 42^T, was isolated from soil collected from Mount Song, Henan Province, China, and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the organism should be assigned to the genus Lentzea and had the closest relationship with Lentzea soli NEAU-LZC 7^T (99.1 % similarity) and Lentzea cavernae SYSU K10001^T (98.2 %). The major menaquinones were identified as MK-9(H4) and MK-9(H2). The phospholipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, glycophospholipid and three unidentified lipids. The major fatty acids were iso-C16 : 0, C16 : 1ω7c and C16 : 0. DNA-DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-LZS 42^T could be clearly differentiated from L. soli NEAU-LZC 7^T and L. cavernae SYSU K10001^T. Therefore, it is concluded that strain NEAU-LZS 42^T represents a novel species of the genus Lentzea, for which the name Lentzea terrae sp. nov. is proposed. The type stain is NEAU-LZS 42^T (=CGMCC 4.7428^T=DSM 105696^T).

Keywords: 16S rRNA gene; Lentzea terrae sp. nov.; polyphasic analysis.