Instrumentation for Vibrational Circular Dichroism Spectroscopy: Method Comparison and Newer Developments

Molecules. 2018 Sep 19;23(9):2404. doi: 10.3390/molecules23092404.

Abstract

Vibrational circular dichroism (VCD) is a widely used standard method for determination of absolute stereochemistry, and somewhat less so for biomolecule characterization and following dynamic processes. Over the last few decades, different VCD instrument designs have developed for various purposes, and reliable commercial instrumentation is now available. This review will briefly survey historical and currently used instrument designs and describe some aspects of more recently reported developments. An important factor in applying VCD to conformational studies is theoretical modeling of spectra for various structures, techniques for which are briefly surveyed.

Keywords: IR polarization optics; absolute configuration; biopolymer secondary structure; instrument design; vibrational circular dichroism.

Publication types

  • Review

MeSH terms

  • Circular Dichroism / instrumentation*
  • Circular Dichroism / methods*
  • Molecular Conformation*
  • Signal Processing, Computer-Assisted / instrumentation*
  • Stereoisomerism*