An electrochemical biosensor based on hemoglobin-oligonucleotides-modified electrode for detection of acrylamide in potato fries

Maryam Asnaashari; Reza Esmaeilzadeh Kenari; Reza Farahmandfar; Khalil Abnous; Seyed Mohammad Taghdisi

doi:10.1016/j.foodchem.2018.07.150

An electrochemical biosensor based on hemoglobin-oligonucleotides-modified electrode for detection of acrylamide in potato fries

Food Chem. 2019 Jan 15:271:54-61. doi: 10.1016/j.foodchem.2018.07.150. Epub 2018 Jul 24.

Authors

Maryam Asnaashari¹, Reza Esmaeilzadeh Kenari¹, Reza Farahmandfar¹, Khalil Abnous², Seyed Mohammad Taghdisi³

Affiliations

¹ Department of Food Science and Technology, Sari Agricultural Sciences & Natural Resources University (SANRU), Sari, Iran.
² Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: abnouskh@mums.ac.ir.
³ Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: taghdisihm@mums.ac.ir.

PMID: 30236713
DOI: 10.1016/j.foodchem.2018.07.150

Abstract

Acrylamide a neurotoxin and strong carcinogen, is found in various thermally processed foods. In this study, an electrochemical sensor for detection of acrylamide using double stranded DNA (dsDNA)/Hemoglobin (Hb)-modified screen printed gold electrode (SPGE) was designed. The immobilization of ssDNA1-SH on the surface of SPGE was confirmed by cyclic voltammetry, and the interaction between ssDNA2-NH₂ and Hb with the ratio 1:1 was characterized by agarose gel. The excellent response of the designed biosensor towards acrylamide due to acrylamide and Hb adducts and change of reduction/oxidation process of Hb-Fe(III)/Hb-Fe(II) was determined by square wave voltammetry (SWV). The biosensor showed the optimum response at pH 8.0. The linear working range for acrylamide was from 2.0 × 10^-6 to 5.0 × 10^-2 M with a detection limit of 1.58 × 10^-7 M. The biosensor was suitable for direct determination of acrylamide in water extracted of potato fries and displayed good reproductivity and high stability.

Keywords: Acrylamide; Biosensor; Hemoglobin; Oligonucleotides; Potato fries; Screen printed gold electrode.

MeSH terms

Acrylamide / chemistry*
Acrylamide / isolation & purification
Biosensing Techniques / methods*
Electrochemical Techniques / methods*
Electrodes
Ferric Compounds
Food Contamination / analysis*
Hemoglobins
Oligonucleotides
Solanum tuberosum / chemistry*

Substances

Ferric Compounds
Hemoglobins
Oligonucleotides
Acrylamide