Simultaneous determination of tocols, γ-oryzanols, phytosterols, squalene, cholecalciferol and phylloquinone in rice bran and vegetable oil samples

Piramon Pokkanta; Phumon Sookwong; Manatchanok Tanang; Saranya Setchaiyan; Pittayaporn Boontakham; Sugunya Mahatheeranont

doi:10.1016/j.foodchem.2018.07.225

Simultaneous determination of tocols, γ-oryzanols, phytosterols, squalene, cholecalciferol and phylloquinone in rice bran and vegetable oil samples

Food Chem. 2019 Jan 15:271:630-638. doi: 10.1016/j.foodchem.2018.07.225. Epub 2018 Aug 1.

Authors

Piramon Pokkanta¹, Phumon Sookwong², Manatchanok Tanang³, Saranya Setchaiyan³, Pittayaporn Boontakham³, Sugunya Mahatheeranont⁴

Affiliations

¹ Rice and Cereal Chemistry Research Laboratory, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: piramon_po@cmu.ac.th.
² Rice and Cereal Chemistry Research Laboratory, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center on Chemistry for Development of Health Promoting Products from Northern Resources, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: phumon.s@cmu.ac.th.
³ Rice and Cereal Chemistry Research Laboratory, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
⁴ Rice and Cereal Chemistry Research Laboratory, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center on Chemistry for Development of Health Promoting Products from Northern Resources, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: sugunya.m@cmu.ac.th.

PMID: 30236726
DOI: 10.1016/j.foodchem.2018.07.225

Abstract

In this study, a simultaneous analytical method of tocols, γ-oryzanols, phytosterols, squalene, cholecalciferol and phylloquinone were developed using HPLC-DAD-FLD. The developed method allowed the quantification of 18 compounds in 30 min. Method validation showed linearity of calibration curves (α = 0.05). RSD of intra-day, inter-day and inter-laboratory precision were less than 4.88%. The limit of detections (LODs) and limit of quantifications (LOQs) were low (0.009-2.166 μg g^-1) with recoveries around 96.0-102.9%. Results derived from the established method demonstrated a wide variation of detected compounds in rice bran and vegetable oil samples (22.4-1774.6 μg g^-1 tocols, ND-26484 μg g^-1 γ-oryzanols, ND-12655 μg g^-1 phytosterols, ND-3189 μg g^-1 squalene, ND-105.3 μg g^-1 cholecalciferol, and ND-54.4 μg g^-1 phylloquinone). Thus, the developed HPLC-DAD-FLD method is a powerful analytical tool for the above mentioned compounds useful in food and pharmaceutical application.

Keywords: Antioxidants; Campesterol (PubChem CID: 173183); Campesteryl ferulate (PubChem CID: 15056832); Cholecalciferol (PubChem CID: 5280795); Cycloartenyl ferulate (PubChem CID: 5282164); HPLC; Lipids; Oil; Phylloquinone (PubChem CID: 5284607); Rice; Simultaneous analysis; Squalene (PubChem CID: 638072); Stigmasterol (PubChem CID: 5280794); α-Tocopherol (PubChem CID: 14985); α-Tocotrienol (PubChem CID: 5282347); β-Sitosterol (PubChem CID: 222284); β-Sitosteryl ferulate (PubChem CID: 9938436); β-Tocopherol (PubChem CID: 6857447); β-Tocotrienol (PubChem CID: 5282348); γ-Tocopherol (PubChem CID: 14986); γ-Tocotrienol (PubChem CID: 5282349); δ-Tocopherol (PubChem CID: 92094); δ-Tocotrienol (PubChem CID: 5282350).

MeSH terms

Cholecalciferol / analysis
Oryza / chemistry*
Phenylpropionates / analysis
Phytosterols / analysis*
Plant Oils / chemistry*
Squalene / analysis
Vitamin K 1

Substances

Phenylpropionates
Phytosterols
Plant Oils
Cholecalciferol
Squalene
Vitamin K 1
gamma-oryzanol