Abstract
S-Adenosylhomocysteine was found to have no effect on Epstein-Barr virus-induced transformation of B-lymphocytes and to stimulate viral capsid antigen expression only slightly in the FF41-1 cell line. In contrast, the S-adenosylhomocysteine analogs sinefungin and S-isobutyladenosine inhibited Epstein-Barr virus transformation and induced a significant increase in the numbers of cells expressing the viral capsid antigen. An inverse relationship between levels of viral DNA methylation and gene expression was demonstrated.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Capsid / genetics*
-
Cell Line
-
Cell Transformation, Viral / drug effects*
-
DNA, Viral / genetics
-
DNA, Viral / metabolism*
-
Herpesvirus 4, Human / drug effects
-
Herpesvirus 4, Human / genetics*
-
Homocysteine / analogs & derivatives*
-
Humans
-
Lymphocytes / cytology
-
Lymphocytes / microbiology
-
Methylation
-
S-Adenosylhomocysteine / analogs & derivatives*
-
S-Adenosylhomocysteine / pharmacology*
-
Structure-Activity Relationship
Substances
-
DNA, Viral
-
Homocysteine
-
S-Adenosylhomocysteine