Abstract
Restriction enzyme maps were made of the region around exons 1 and 2 of activated c-Ki-ras of a fibrosarcoma (1,8-DNP2) induced in a rat by 1,8-dinitropyrene. Nucleotide sequence analysis revealed that activated c-Ki-ras shows a G----T transversion in codon 12 and consequently encodes cysteine instead of glycine in normal rat c-Ki-ras.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Carcinogens / pharmacology*
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Cell Transformation, Neoplastic*
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Cells, Cultured
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DNA Restriction Enzymes
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Fibrosarcoma / genetics*
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Mice
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Mice, Inbred Strains
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Oncogenes / drug effects*
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Pyrenes / pharmacology*
Substances
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Carcinogens
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Pyrenes
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1,8-dinitropyrene
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DNA Restriction Enzymes
Associated data
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GENBANK/M12259
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GENBANK/M12260