Structure of the c-Ki-ras gene in a rat fibrosarcoma induced by 1,8-dinitropyrene

Mol Cell Biol. 1986 Apr;6(4):1349-51. doi: 10.1128/mcb.6.4.1349-1351.1986.

Abstract

Restriction enzyme maps were made of the region around exons 1 and 2 of activated c-Ki-ras of a fibrosarcoma (1,8-DNP2) induced in a rat by 1,8-dinitropyrene. Nucleotide sequence analysis revealed that activated c-Ki-ras shows a G----T transversion in codon 12 and consequently encodes cysteine instead of glycine in normal rat c-Ki-ras.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carcinogens / pharmacology*
  • Cell Transformation, Neoplastic*
  • Cells, Cultured
  • DNA Restriction Enzymes
  • Fibrosarcoma / genetics*
  • Mice
  • Mice, Inbred Strains
  • Oncogenes / drug effects*
  • Pyrenes / pharmacology*

Substances

  • Carcinogens
  • Pyrenes
  • 1,8-dinitropyrene
  • DNA Restriction Enzymes

Associated data

  • GENBANK/M12259
  • GENBANK/M12260