Ethyl β-d-glucoside: a novel chemoattractant of Ralstonia solanacearum isolated from tomato root exudates by a bioassay-guided fractionation

Biosci Biotechnol Biochem. 2018 Dec;82(12):2049-2052. doi: 10.1080/09168451.2018.1515618. Epub 2018 Sep 21.

Abstract

A chemoattractant of Ralstonia solanacearum isolated from the activated charcoal-adsorbed fraction of tomato root exudates was identified as ethyl β-d-glucopyranoside by instrumental analyses and comparison with synthetic preparations. Ethyl β-D-glucopyranoside showed unambiguous activity at above 1 µmol/disc. Its stereoisomers and D-glucose were inactive.

Keywords: Ralstonia solanacearum; bacterial wilt; chemotaxis; root exudates; tomato.

MeSH terms

  • Biological Assay
  • Carbon-13 Magnetic Resonance Spectroscopy
  • Chemical Fractionation / methods*
  • Chemotactic Factors / chemistry
  • Chemotactic Factors / isolation & purification*
  • Chemotactic Factors / pharmacology*
  • Chromatography, High Pressure Liquid
  • Glucosides / chemistry
  • Glucosides / isolation & purification
  • Glucosides / pharmacology*
  • Plant Exudates / chemistry*
  • Plant Roots / chemistry*
  • Proton Magnetic Resonance Spectroscopy
  • Ralstonia solanacearum / drug effects*
  • Solanum lycopersicum / chemistry*

Substances

  • Chemotactic Factors
  • Glucosides
  • Plant Exudates